Differential rescue of the MIIB-induced polarity defect by the domain swap chimeras. MIIB knockdown cells were rescued with the indicated myosin II chimeras and plated on fibronectin. The polarity index of CHO.K1 cells was calculated as in Fig. 4. Data represent the mean ± SD of >100 cells analyzed per condition in four independent experiments. Bar, 10 μm. *, Student's t test of MLC-DD+GFP-MIIB/A vs. rescue with MIIB.