MIIB creates a cellular tail. (A) Representative images of a control or MIIB-deficient CHO.K1 cells, coexpressing control GFP-actin, RNAi-insensitive GFP-MIIB, and MLC-WT-GFP or MLC-DD-GFP. Note that MLC-DD does not rescue the polarity loss induced by depletion of MIIB. Bar, 10 μm. (B) Polarity index of CHO.K1 cells in the conditions shown in A. Data represent the mean ± SD of >100 cells analyzed per condition in four independent experiments. *, Student's t test of MLC-WT and MLC-DD vs. control.