RhoA and mDia1 restore MT dynamics and peripheral adhesions in Memo-depleted cells. (A and B) SKBR3 cells expressing Memo, mDia1, or control siRNA and constructs coding for Memo, active mDia1 (mDia1[ΔN3]), or active RhoA (RhoAV14) were analyzed for MT dynamic instability as in Fig. 1. (B) Top left, catastrophes; top right, rescues. Values are means ± SD from three independent experiments (30 MTs and nine cells). *, P < 0.01 by permutation test. (C) Formation of adhesion sites was analyzed as in Fig. 2 A. Where indicated, cells were treated with 10 μM Y27632 for 120 min before addition of HRG. Bars, 10 μm.