Figure 4.
Figure 4. Immunoelectron microscopy localization of GFP-Rab7 and Vps26. Ultrathin cryosections of HeLa cells expressing wild-type GFP-Rab7 were immunogold labeled with antibodies to GFP (10-nm gold particles) and Vps26 (15-nm gold particles). Arrows indicate the presence of GFP-Rab7 on buds adjacent to the vacuolar part of endosomes (A) and on tubules (B). Tubules and buds are also labeled for Vps26. Bars, 200 nm.

Immunoelectron microscopy localization of GFP-Rab7 and Vps26. Ultrathin cryosections of HeLa cells expressing wild-type GFP-Rab7 were immunogold labeled with antibodies to GFP (10-nm gold particles) and Vps26 (15-nm gold particles). Arrows indicate the presence of GFP-Rab7 on buds adjacent to the vacuolar part of endosomes (A) and on tubules (B). Tubules and buds are also labeled for Vps26. Bars, 200 nm.

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