APCs are not required for axon outgrowth and fasciculation. (A) Cartoon of axon projections, CB NBs (left), and lobullar plug neurons (right). (B–N) Third instar brains, genotypes, and antigens as indicated. (B) Drosophila E-cadherin enriched in CB NB, neurons, and axons (yellow arrow). (C) APC2g10APC1Q8 clone in the context of the brain lobe. Axons, yellow arrow. (D–M) CB NB clones, clonal cells marked with CD8-GFP. (E–J) Red arrows, CB NBs; yellow arrows or arrowheads, axons of progeny of particular NBs; asterisks, GFP-labeled clones in other cell types. (J) Cross sections of fasciculated axons of several CB NBs; yellow arrows, APC2g10APC1Q8 mutant clone; white arrows, wild type. (K–M). Wild-type or mutant clones of lobular plug neurons. (L) Purple arrow indicates the axon target in laminar neuropil. Bars, 10 μm.
