CB NBs do not require APCs for persistent spindle positioning. (A–C) Time-lapse images; NB clones followed for two rounds of division. Tau∷GFP (A and B) or CD8∷GFP (C). Arrowheads, GMCs from first round of division; arrows, GMCs from second round. (A) Control clone. (B) APC2^d40APC1^Q8 clone. (C) APC2^g10 APC1^Q8 clone. The GMC from the first round does not remain in focus during the second round. (D–H) Bent spindle phenotype. (D and E) Fixed samples. (D) Wild type (WT). (E) APC2^g10APC1^Q8. (F–H) Time lapse of NB clones, prophase, metaphase, and one frame before anaphase onset (AO). (F) Control. (G) APC2^d40APC1^Q8 transitioning through bent spindle intermediate. Dashed circles outline NB. (H) APC2^d40APC1^Q8 without bent spindle. Time is given in hours:minutes:seconds. Bars,10 μm.