CB NBs do not require APCs for asymmetric cell division. (A) Larval brain. (B) CB NB, GMCs, neurons, and their fasciculated axons and target. (C) Asymmetric APC2 localization in CB NB (arrow). (D–G) Time-lapse images, wild-type, or double mutant NBs from nuclear envelope breakdown through next interphase. In all panels, NB divides asymmetrically (fourth column, brackets). White arrows, contractile ring; yellow arrows, new GMC. (D) Wild-type control clone. CD8∷GFP marks cell membrane, nuclei, and spindle. (inset) Different plane of focus showing nuclear envelope. (E) APC2^g10 APC1^Q8, CD8∷GFP. (F) Control clone, Tau∷GFP marking MTs. (G) APC2^d40 APC1^Q8, Tau∷GFP. Time is given in minutes:seconds. Bars, 10 μm.