A Survivin linker mutant localizes aberrantly. (A) Survivin^L98A/V100A-GFP fails to localize at 39°C in the presence of wild-type rescue Survivin (a, c, and e). In the absence of wild-type rescue Survivin, a subset of Survivin^L98A/V100A-GFP localizes normally (b, d, and f). INCENP localizes normally in all cells. (B) Survivin^L98A/V100A-GFP fails to localize at 41°C in the presence or absence of wild-type rescue Survivin. INCENP localizes normally only in the presence of wild-type rescue Survivin (a, c, and e). (C) Summary of the mutational analysis of Survivin. Bars, 5 μm.