Survivin linker mutants are temperature sensitive. (A) Location of L98, V100, L104, and L106 in the CPC. (B) Expression of Survivin^L98A/V100A-GFP and Survivin^L104A/L106A-GFP and loss of wild-type (WT) Survivin expression from cultures grown in doxycycline at 39 or 41°C. (C) Survivin^L98A/V100A-GFP rescues the life of Survivin^OFF cells at 39°C. (D) Survivin^L104A/L106A-GFP rescues the life of Survivin^OFF cells at 37°C. (E and F) Cells expressing solely Survivin^L98A/V100A-GFP and Survivin^L104A/L106A-GFP die at 41°C, albeit with slightly delayed kinetics.