Smac and aurora B binding are not essential for Survivin function. (A) Location of D55 in the Survivin dimer. (B) Expression of Survivin^D55A-GFP and loss of wild-type (WT) Survivin expression from cultures grown in doxycycline. (C) Survivin^D55A-GFP rescues the life of Survivin^OFF cells. (D) Survivin^D55A-GFP colocalizes normally with INCENP in Survivin^ON/OFF cells. (E) Location of D72 and D73 in the Survivin dimer. (F) Expression of Survivin^D72A/D73A-GFP and loss of wild-type Survivin expression from cultures grown in doxycycline. (G) Survivin^D72A/D73A-GFP fails to localize in Survivin^ON cells; INCENP localization is normal (a and b). Survivin^D72A/D73A-GFP fails to localize in Survivin^OFF cells at prometaphase and metaphase; INCENP localization at centromeres is also compromised (c). Survivin^D72A/D73A-GFP and INCENP localize normally in Survivin^OFF cells at anaphase/telophase (d). (H) Survivin^D72A/D73A-GFP rescues the life of Survivin^OFF cells. Bars, 5 μm.