Functional analysis of Survivin protein in Survivin^ON/OFF cells. (A) Alignment of human and chicken Survivin sequences showing conserved residues mutated in this study. (B and C) Chicken (g) and human (h) Survivin-GFP rescues the life of Survivin^OFF cells. Growth curves for cells expressing GFP fusion proteins are green. (D and E) Chicken and human Survivin-GFP colocalize normally with INCENP (red) in Survivin^OFF cells. (F) The location of residue T36 in the CPC and dimer structures of Survivin (Chantalat et al., 2000; Jeyaprakash et al., 2007). (G) Expression of Survivin^T36A/E-GFP and loss of wild-type (WT) Survivin expression from cultures grown in doxycycline. (H) Chicken Survivin^T36A-GFP rescues the life of Survivin^OFF cells. (I) gSurvivin^T36A-GFP colocalizes normally with INCENP in Survivin^ON/OFF cells. Bars, 5 μm.