Cells lacking Survivin die in interphase after failing to complete cytokinesis. (A) Selected frames from a video in which a Survivin^OFF cell fails to complete cytokinesis and dies by apoptosis in the subsequent interphase. Time is given in hours/minutes. Bar, 10 μm. (B) Analysis of cell death in Survivin^OFF cells from videos like those shown in A. Bars begin at anaphase onset (open circles) and terminate either at cell death (closed circles) or at the end of the video. Bar M shows the mean length of mitosis in DT40 (30 min). (C) Survivin^OFF cells exhibit a normal mitotic arrest in nocodazole but not in 10 nM taxol. (D) Survivin^OFF cells arrest in mitosis at taxol concentrations ≥100 nM. (E) The death of Survivin^OFF cells is exacerbated by 10 nM but not by 200 nM taxol. (F) Survivin^OFF and Survivin^ON control cultures exhibit similar levels of apoptotic death after exposure to 10 μM etoposide. Values for cells grown in doxycycline are shown as striped bars. Error bars indicate SD. WT, wild type.