Reduction of DE-cadherin function or adhesion between germ cells does not rescue the tre1 phenotype. (A and B) In tre1 mutants, germ cells (brown) form a tight cluster inside the midgut (white dotted lines in A–F, J, and K) during stages 8–11. (C and D) In tre1 and maternal shg^A9-49 double mutants, germ cells disperse within the midgut (C, arrow); however, they are not able to migrate through the midgut (D, arrow). (E and F) Single germ cells in maternal tud^A36-38 mutants cross the midgut normally (E, arrow) to reach the gonad (F, yellow dotted circle). (G–I) Polarity of single germ cells. Maternal tud^A36-38 mutant embryos were stained with anti-Vasa (green) and anti–DE-cadherin antibodies (red). Single germ cells display a polarized morphology with a tail at stage 9. Vasa channel is shown in H, and the DE-cadherin channel is shown in I. (J and K) Single germ cells (arrows) in tre1 and maternal tud^A36-38 double mutants fail to migrate through the midgut. Embryos are oriented anterior to the left, lateral view in all panels, except for embryos in A and C, which are oriented dorsally. Bars: (G) 20 μm; (K) 50 μm.