tre1 regulates DE-cadherin localization in the tail of germ cells. (A–F) DE-cadherin localization in wild-type and tre1 germ cells at stage 5. Embryos were stained with anti–DE-cadherin (red) and anti-Vasa antibodies (green). DE-cadherin is distributed uniformly in the germ cell membrane in the wild type (A–C) and tre1 mutants (D–F). (G–L) DE-cadherin localization in wild-type and tre1 germ cells at stage 9. In wild-type germ cells, DE-cadherin is enriched in the tail region (G–I). In tre1 mutant germ cells, DE-cadherin is evenly distributed in the cell membrane (J–L). Vasa channel is shown in B, E, H, and K, and the DE-cadherin channel is shown in C, F, I, and L. (M) Quantification of DE-cadherin levels in the cell body membrane of stage 5 and 9 germ cells. Normalized intensity of the DE-cadherin staining is shown (see Materials and methods). DE-cadherin levels are similar in wild-type and tre1 germ cells at stage 5. At stage 9, DE-cadherin levels in tre1 mutant germ cells are significantly higher than wild-type cells. Error bars indicate standard error. Bar, 20 μm.