Spindle and kinetochore morphology of HeLa MUG. (A and B) Differential interference contrast (A) and centrin1-GFP (B) images of a fixed cell during MUG metaphase. (C–F) This cell was processed for correlative EM. (C) A single lower magnification section through the spindle. Note that chromatin is largely absent from the spindle except for a few small fragments (arrows). (D–F) 70-nm-thick serial sections through a kinetochore. Microtubules attach to the kinetochore end-on as well as laterally (E, arrow).