Figure 2.
Interaction of the Ndc80/Nuf2 dimer with the microtubule lattice. (A) An example of a vitrified microtubule decorated with the Ndc80/Nuf2 dimer. (B) A typical diffraction pattern (positions of 4- and 8-nm layer lines are marked). (C) A projection view of the three-dimensional map. (D) A magnified view of the boxed area in the three-dimensional map in C confirms the binding of the Ndc80/Nuf2 dimer to both α- and β-tubulin. Arrows point to the protruding density of the Ndc80/Nuf2 dimer in A and D. + and − signs indicate microtubule polarity. Bar, 85 nm.