Figure 5. Knocking-in a wild-type, but... | Rockefeller University Press

Figure 5.

$Figure 5. Knocking-in a wild-type, but not mCBM α1 mutant rescues Cav1 distribution in TCN23-19 cells. TCN23-19 cells were rescued by YFP-tagged wild-type α1 (wt α1) or mCBM. Stable cell lines were generated and analyzed as described in Fig. 1. (A) A set of representative Western blots from four independent experiments showing the expression of both YFP-α1/mCBM α1 (140 kD) and residual endogenous α1 (110 kD), and the distribution of Cav1 in each fraction. (B) Combined data from four different experiments shows that expression of wild-type rat α1, but not the mCBM α1, restored the distribution of Cav1 into the 4/5 fraction in TCN23-19 cells . **, P < 0.01 in comparison with control P-11. Endo α1: endogenous α1.$

Knocking-in a wild-type, but not mCBM α1 mutant rescues Cav1 distribution in TCN23-19 cells. TCN23-19 cells were rescued by YFP-tagged wild-type α1 (wt α1) or mCBM. Stable cell lines were generated and analyzed as described in Fig. 1. (A) A set of representative Western blots from four independent experiments showing the expression of both YFP-α1/mCBM α1 (140 kD) and residual endogenous α1 (110 kD), and the distribution of Cav1 in each fraction. (B) Combined data from four different experiments shows that expression of wild-type rat α1, but not the mCBM α1, restored the distribution of Cav1 into the 4/5 fraction in TCN23-19 cells . **, P < 0.01 in comparison with control P-11. Endo α1: endogenous α1.