Figure 4.
Figure 4. FRET analyses of the interaction between Cav1 and Na/K-ATPase α1 subunit. YFP- tagged α1, D371N, or mCBM was transfected together with CFP-Cav1 into TCN23-19 cells. FRET analyses were done as described in Materials and methods. YFP-only and CFP-Cav1–transfected cells were used as a negative control. (A) Images were taken before and after photobleaching. The ROI_1 was bleached at 515 nm with full power. Small regions of plasma membrane in (ROI_2) and out (ROI_3) of the bleached area were selected for measurement, and FRET efficiency was calculated from the measurements using the equation as shown in the table. Bar, 2 μm. (B) Corrected FRET efficiency was calculated from each experiment and values are mean ± SE of 4–5 independent experiments. *, P < 0.05 in comparison to control.

FRET analyses of the interaction between Cav1 and Na/K-ATPase α1 subunit. YFP- tagged α1, D371N, or mCBM was transfected together with CFP-Cav1 into TCN23-19 cells. FRET analyses were done as described in Materials and methods. YFP-only and CFP-Cav1–transfected cells were used as a negative control. (A) Images were taken before and after photobleaching. The ROI_1 was bleached at 515 nm with full power. Small regions of plasma membrane in (ROI_2) and out (ROI_3) of the bleached area were selected for measurement, and FRET efficiency was calculated from the measurements using the equation as shown in the table. Bar, 2 μm. (B) Corrected FRET efficiency was calculated from each experiment and values are mean ± SE of 4–5 independent experiments. *, P < 0.05 in comparison to control.

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