Figure 1.
Figure 1. ER tubules bind and collapse on chromatin in vivo. U2OS cells were transfected with GFP fusion proteins and H2B-tdTomato, then imaged through mitosis with real-time spinning-disk confocal microscopy. (A) Large images of the initial interactions of Sec61-GFP and POM121-3GFP are shown. (B) z stacks were acquired every 30 s and initial membrane–chromatin contact points were characterized by 3D reconstruction. Arrowheads indicate initial membrane–chromatin contact. (C) Sec61-GFP was imaged every 5 s to monitor ER dynamics. (D) POM121-3GFP was imaged every 10 s to monitor the redistribution from the ER to the forming NE. Bars, 10 μm.

ER tubules bind and collapse on chromatin in vivo. U2OS cells were transfected with GFP fusion proteins and H2B-tdTomato, then imaged through mitosis with real-time spinning-disk confocal microscopy. (A) Large images of the initial interactions of Sec61-GFP and POM121-3GFP are shown. (B) z stacks were acquired every 30 s and initial membrane–chromatin contact points were characterized by 3D reconstruction. Arrowheads indicate initial membrane–chromatin contact. (C) Sec61-GFP was imaged every 5 s to monitor ER dynamics. (D) POM121-3GFP was imaged every 10 s to monitor the redistribution from the ER to the forming NE. Bars, 10 μm.

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