TLR-induced podosome disassembly requires a distinct metalloprotease activity. DC from MT1-MMP– (A) and MMP2- or MMP9- (B) deficient mice were fixed after LPS stimulation (50 ng/ml, 30 min) and stained with TRITC-phalloidin to allow assessment of podosome loss. SDC were pretreated for 20 min with 25 μM TAPI-1, 3 μM GW280264X (GW), or 3 μM GI254023X (GI), then stimulated with 100 ng/ml Pam3CSK. TNF-α shedding into the medium measured by ELISA (C) and quantitation of cells with podosomes (D) are shown. Data are representative of experiments performed on DC from at least two mice.