Figure 4.
Figure 4. MT1-MMP–deficient DC are unable to degrade matrix. DC from wild-type or MMP-deficient mice were plated on cross-linked FITC-gelatin matrix for 4 h or for the times indicated, then fixed and stained with TRITC-phalloidin. (A) Quantitation of matrix degradation by DC from MMP2−/− and MMP9−/− mice. (B) Confocal images of MT1-MMP+/+ and MT1-MMP−/− SDC plated on matrix. Bars 20 μm. (C) Quantitation of matrix degradation by MT1-MMP+/+ and MT1-MMP−/− BMDC and SDC. Data are means of experiments performed on DC cultures from two mice of each genotype (error bars indicate the data range), and are representative of data from five MT1-MMP−/− mice in total. (D) The percentage of MT1-MMP+/+ and MT1-MMP−/− BMDC and SDC with podosomes when plated on matrix.

MT1-MMP–deficient DC are unable to degrade matrix. DC from wild-type or MMP-deficient mice were plated on cross-linked FITC-gelatin matrix for 4 h or for the times indicated, then fixed and stained with TRITC-phalloidin. (A) Quantitation of matrix degradation by DC from MMP2−/− and MMP9−/− mice. (B) Confocal images of MT1-MMP+/+ and MT1-MMP−/− SDC plated on matrix. Bars 20 μm. (C) Quantitation of matrix degradation by MT1-MMP+/+ and MT1-MMP−/− BMDC and SDC. Data are means of experiments performed on DC cultures from two mice of each genotype (error bars indicate the data range), and are representative of data from five MT1-MMP−/− mice in total. (D) The percentage of MT1-MMP+/+ and MT1-MMP−/− BMDC and SDC with podosomes when plated on matrix.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal