Figure 1.
Figure 1. TLR-signaling induces an increase in cell spreading and a switch from podosomes to focal contacts. SDC were treated with 50 ng/ml LPS (A) or 100 ng/ml Pam3CSK (B) for the times indicated, then stained for F-actin (red) and vinculin (green). Arrows indicate focal adhesion–like contacts. The number of focal contacts per cell (C), the percentage of cells with focal contacts (D), and the ventral cell surface area of each cell (E) were quantitated from confocal images of untreated cells and cells treated with 100 ng/ml Pam3CSK for 30 min. Approximately 50 cells for each condition were quantitated for C. At least 450 cells for D and 120 cells for E for each condition, from three experiments performed on independent DC cultures, were scored. Data are means ± SEM. *, P < 0.005. Bars: (A) 10 μm; (B) 20 μm.

TLR-signaling induces an increase in cell spreading and a switch from podosomes to focal contacts. SDC were treated with 50 ng/ml LPS (A) or 100 ng/ml Pam3CSK (B) for the times indicated, then stained for F-actin (red) and vinculin (green). Arrows indicate focal adhesion–like contacts. The number of focal contacts per cell (C), the percentage of cells with focal contacts (D), and the ventral cell surface area of each cell (E) were quantitated from confocal images of untreated cells and cells treated with 100 ng/ml Pam3CSK for 30 min. Approximately 50 cells for each condition were quantitated for C. At least 450 cells for D and 120 cells for E for each condition, from three experiments performed on independent DC cultures, were scored. Data are means ± SEM. *, P < 0.005. Bars: (A) 10 μm; (B) 20 μm.

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