IL-2 production is impaired by p50-dynamitin-GFP overexpression and DHC interference. (A) CD69 induction was detected by flow cytometry in J77 Jurkat clones overexpressing GFP or p50-dynamitin-GFP (c) and conjugated with unprimed or SEE-pulsed Raji APC for 16 h (percentage of cells is shown). Three experiments were performed in triplicate. (B and C) IL-2 was detected by ELISA in culture supernatants (B) or cell lysates (C) from the cultures in A. Three experiments were performed in triplicate. (D) IL-2 detected by ELISA in cell supernatants from DHC-silenced J77 cells (siDHC, closed bars) and controls (siNeg, open bars) conjugated with SEE-pulsed Raji APC with the indicated doses (μg/ml). Three experiments were performed in triplicate. Data are means ± SD. *, P < 0.05; **, P < 0.001 (Student's t test).