TCR-sustained signaling is impaired by RNA interference of DHC (A and B) Western analysis of signaling proteins in conjugates formed for the times indicated between DHC silenced T cells (siDHC) or controls (siNeg) and activated APC. T cell–APC pairs were J77 cells and SEE-pulsed Raji cells (0.3 μg/ml; A) and CH7C17 cells and HA peptide-preloaded Hom2 cells (50 μg/ml; B). pY783 PLCγ1, PLCγ1 phosphorylated on Y783; all other kinases are as in Fig. 4 (A–E). One out of four representative experiments is shown. Tubulin is shown as additional loading control. Arrows indicate target band sizes.