Figure 2.
Figure 2. When RPE-1 cells cannot satisfy the SAC, the formation of spindle MTs does not accelerate mitotic slippage. (A and B) Selected images from video recordings of cells entering and exiting mitosis in the presence of 500 nM taxol (A), which stabilizes spindle MTs, or 2.5 μM of the Eg5 inhibitor dimethylenastron (B). In each sequence the second frame defines the start of mitosis, the third the end, and the last depicts the chromosome (DNA) and MT pattern in a mitotic cell from a similarly treated culture fixed and stained for the immunofluorescence localization of MTs. Time from addition of drug to the medium is in minutes. See text for details. Arrows note the cell that is followed in subsequent frames. Bars: (IMF images) 5 μm; (phase images) 20 μm.

When RPE-1 cells cannot satisfy the SAC, the formation of spindle MTs does not accelerate mitotic slippage. (A and B) Selected images from video recordings of cells entering and exiting mitosis in the presence of 500 nM taxol (A), which stabilizes spindle MTs, or 2.5 μM of the Eg5 inhibitor dimethylenastron (B). In each sequence the second frame defines the start of mitosis, the third the end, and the last depicts the chromosome (DNA) and MT pattern in a mitotic cell from a similarly treated culture fixed and stained for the immunofluorescence localization of MTs. Time from addition of drug to the medium is in minutes. See text for details. Arrows note the cell that is followed in subsequent frames. Bars: (IMF images) 5 μm; (phase images) 20 μm.

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