Figure 2.
Figure 2. SopB mediates PI3-kinase activation at the plasma membrane during Salmonella invasion. (A) Lipids were extracted and analyzed by HPLC. The amount of phosphoinositides is given as the percentage of PI in the same sample. PI(4/5)P refers to the sum of PI(4)P and PI(5)P. (B–E) Insets are provided (arrows) for each 3′-phosphorylated phosphoinositide. Data are means ± SEM of five separate experiments. Levels of significance are indicated by p-values. (F and G) Cells were transfected with PH(Akt)-GFP and infected with WT (F) or sopB mutant (G). Cells were analyzed using confocal microscopy. Left panels indicate the PH(Akt)-GFP localization relative to the RFP- expressing bacteria (red) in the merged images in the right panels. Insets are enlarged from dashed boxes. These images correspond to <15 min of infection. Bars, 10 μm.

SopB mediates PI3-kinase activation at the plasma membrane during Salmonella invasion. (A) Lipids were extracted and analyzed by HPLC. The amount of phosphoinositides is given as the percentage of PI in the same sample. PI(4/5)P refers to the sum of PI(4)P and PI(5)P. (B–E) Insets are provided (arrows) for each 3′-phosphorylated phosphoinositide. Data are means ± SEM of five separate experiments. Levels of significance are indicated by p-values. (F and G) Cells were transfected with PH(Akt)-GFP and infected with WT (F) or sopB mutant (G). Cells were analyzed using confocal microscopy. Left panels indicate the PH(Akt)-GFP localization relative to the RFP- expressing bacteria (red) in the merged images in the right panels. Insets are enlarged from dashed boxes. These images correspond to <15 min of infection. Bars, 10 μm.

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