Figure 1.
Figure 1. Mcm21R is required for efficient bipolar spindle formation in the prometaphase pathway. (A) Percentage of cells undergoing monopolar anaphase when centrosomes are either together or apart at the time of NEBD. (B) Successive frames every 3 min from a live cell video of HeLa cells expressing H2B-EGFP/a-tubulin–mRFP (to mark chromosomes and MTs). Images for α-tubulin–mRFP and the composite images for H2B-EGFP (green) and α-tubulin–mRFP (red) of two adjacent cells are shown. The top cell follows the prophase (P) pathway, and the bottom cell follows the prometaphase (PM) pathway. (C) Percentage of mitotic HeLa cells using either the prophase or prometaphase pathways in siControl (n = 73), siMcm21R (n = 73), siRootletin (n = 31), or siMcm21R + siRootletin (n = 42)–treated cells. (D) Mean bipolar spindle assembly time in the prophase or prometaphase pathway in untreated (n = 69) or treated with siControl (n = 73) or siMcm21R (n = 73) RNAs. (E and F) Successive frames every 3 min from a live cell video of siMcm21R RNA–treated cells in the prophase (E) or prometaphase (F) pathways. (G) Percentage of monopolar spindles in fixed cells after treatment with siControl (n = 90), siMcm21R (n = 90), siRootletin (n = 90), or siMcm21R + siRootletin RNAs (n = 90). Asterisks represent statistically significant differences (P < 0.01 by Mann-Whitney test). Error bars represent SEM. Bars, 10 µm.

Mcm21R is required for efficient bipolar spindle formation in the prometaphase pathway. (A) Percentage of cells undergoing monopolar anaphase when centrosomes are either together or apart at the time of NEBD. (B) Successive frames every 3 min from a live cell video of HeLa cells expressing H2B-EGFP/a-tubulin–mRFP (to mark chromosomes and MTs). Images for α-tubulin–mRFP and the composite images for H2B-EGFP (green) and α-tubulin–mRFP (red) of two adjacent cells are shown. The top cell follows the prophase (P) pathway, and the bottom cell follows the prometaphase (PM) pathway. (C) Percentage of mitotic HeLa cells using either the prophase or prometaphase pathways in siControl (n = 73), siMcm21R (n = 73), siRootletin (n = 31), or siMcm21R + siRootletin (n = 42)–treated cells. (D) Mean bipolar spindle assembly time in the prophase or prometaphase pathway in untreated (n = 69) or treated with siControl (n = 73) or siMcm21R (n = 73) RNAs. (E and F) Successive frames every 3 min from a live cell video of siMcm21R RNA–treated cells in the prophase (E) or prometaphase (F) pathways. (G) Percentage of monopolar spindles in fixed cells after treatment with siControl (n = 90), siMcm21R (n = 90), siRootletin (n = 90), or siMcm21R + siRootletin RNAs (n = 90). Asterisks represent statistically significant differences (P < 0.01 by Mann-Whitney test). Error bars represent SEM. Bars, 10 µm.

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