Figure 7.
Figure 7. Diffusion and dissociation of GAP1IP4BP bound to PtdIns(4,5)P2 ± PtdIns(3,4,5)P3. (A) Domain organization in the primary sequence of GAP1IP4BP and its truncations. The fluorescence micrographs show HEK cells expressing the indicated constructs after 1 h stimulation with 100 nM insulin + 10% serum, 5 µM LY294002, or 10 µM wortmannin. Bar, 10 µm. (inset) The graph shows the ratio of fluorescence intensity at the plasma membrane relative to the cytosol for all cells analyzed. The fitted diffusion coefficients D and membrane dissociation times τ are shown in B and C, respectively. Results of statistical analysis are shown in Tables II and III. Data are means ± SEM.

Diffusion and dissociation of GAP1IP4BP bound to PtdIns(4,5)P2 ± PtdIns(3,4,5)P3. (A) Domain organization in the primary sequence of GAP1IP4BP and its truncations. The fluorescence micrographs show HEK cells expressing the indicated constructs after 1 h stimulation with 100 nM insulin + 10% serum, 5 µM LY294002, or 10 µM wortmannin. Bar, 10 µm. (inset) The graph shows the ratio of fluorescence intensity at the plasma membrane relative to the cytosol for all cells analyzed. The fitted diffusion coefficients D and membrane dissociation times τ are shown in B and C, respectively. Results of statistical analysis are shown in Tables II and III. Data are means ± SEM.

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