Figure 6.
Figure 6. Diffusion of GAP1m in the presence and absence of PtdIns(3,4,5)P3. (A) Fluorescence micrographs of HEK cells expressing GFP-GAP1m after 1 h stimulation with 100 nM insulin in 10% serum, or 5 µM LY294002 in serum-free medium as indicated. Bar, 10 µm. (B) Diffusion coefficients under the above conditions; cytosolic diffusion in the presence of LY294002 is significantly faster than on the membrane in the presence of insulin (P < 0.0001, Mann-Whitney test). Data are means ± SEM.

Diffusion of GAP1m in the presence and absence of PtdIns(3,4,5)P3. (A) Fluorescence micrographs of HEK cells expressing GFP-GAP1m after 1 h stimulation with 100 nM insulin in 10% serum, or 5 µM LY294002 in serum-free medium as indicated. Bar, 10 µm. (B) Diffusion coefficients under the above conditions; cytosolic diffusion in the presence of LY294002 is significantly faster than on the membrane in the presence of insulin (P < 0.0001, Mann-Whitney test). Data are means ± SEM.

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