Dissociation and diffusion of PH-PLCδ1. (A) Fluorescence micrographs of HEK cells expressing single or tandem PH domains from PLCδ1 fused to GFP; where indicated, the domains carry the R40L point mutation in one of the domains. The graph on the right summarizes the ratio of fluorescence intensity at the plasma membrane relative to the cytosol for all cells analyzed, as described in Materials and methods. Bar, 10 µm. (B) Fitted diffusion coefficients; PH-GFP and PH^R40L-GFP are significantly different from each other and the other proteins (P < 0.001, Kruskall-Wallis test with a post hoc Dunn's multiple comparison test). (C) Fitted membrane dissociation time constants τ; GFP-PH-PH and PH-PH-GFP were significantly different from PH-GFP and PH^R40L-PH-GFP (P < 0.001, Kruskall-Wallis test with post hoc Dunn's multiple comparison test). Data are means ± SEM.