BMP-2 facilitates redistribution of Dvl to filopodia and lamellipodia in hPAECs. (A and B) Live images taken over a 10-h period of cells nucleofected with Dvl-GFP (A) or ΔDEP Dvl-GFP (B) and stimulated with either vehicle or 10 ng/ml BMP-2. Arrowheads indicate the marked presence of GFP signal in the migrating front end of Dvl-GFP–transfected cells incubated with BMP-2. See Videos 1–4 (available). (C) The distance and speed of control and BMP-2–stimulated cells transfected with Dvl-GFP and ΔDEP Dvl-GFP. Distance was measured by plotting the coordinates of the nuclei every 10 min for 10 h and adding the distance between the points. Speed was calculated by dividing the distance over time (10 h). Error bars denote mean ± SEM for three different experiments with triplicate assessments. ***, P < 0.001 (vs. baseline). Bars, 10 μm.