Figure 5.
![Figure 5. BMP-2 and Wnt3a increase levels of active RhoA and Rac1 in hPAECs. Active RhoA and Rac1 pull-down experiments were performed on hPAECs starved for 24 h followed by incubation with 10 ng/ml BMP-2 or 100 ng/ml Wnt3a for 1 and 4 h. Representative immunoblots for RhoA and Rac1 are shown along with densitometry. Levels of active RhoA and Rac1 were measured against total RhoA and Rac1 in cell lysates. Error bars denote mean ± SEM for three different experiments with triplicate assessments. *, P < 0.01 and **, P < 0.001 (vs. control [CON]).](https://cdn.rupress.org/rup/content_public/journal/jcb/184/1/10.1083_jcb.200806049/6/jcb_200806049_gs_fig5.jpeg?Expires=1771597772&Signature=HsVfZMQPFg8suIU294eSm9THuZMH7qL8Nzb~hRB~dHgNTQvHiIAczRrvmS9Ead9DGS~GECWu0ABTO2HbCnQS0Xl2sa8u-w93lCBjHbE4biOwCA3LZrvoks9G7Ibgy2~I1AZl9wPO-KhUMRyjltuRAl33vkCWrGk6usTtRh5708PYiqpnJo7V9WM55TtRz6FMAO7EEYr~dk~gCtEgT~-IHFVJE34aLDuu3OZzVXkR~IHGT3agCM5PN0mgJf9YdbRdZqXpJNYhF6c9vwxcErdCdYFtBn9v11xQXwdgITfMGVjHm631zT3Fy2h5okF~EdoMdb20Yaxpp0E2trwyyc1itg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
BMP-2 and Wnt3a increase levels of active RhoA and Rac1 in hPAECs. Active RhoA and Rac1 pull-down experiments were performed on hPAECs starved for 24 h followed by incubation with 10 ng/ml BMP-2 or 100 ng/ml Wnt3a for 1 and 4 h. Representative immunoblots for RhoA and Rac1 are shown along with densitometry. Levels of active RhoA and Rac1 were measured against total RhoA and Rac1 in cell lysates. Error bars denote mean ± SEM for three different experiments with triplicate assessments. *, P < 0.01 and **, P < 0.001 (vs. control [CON]).
