Pxl1 binds the SH3 domain of Cdc15 and functions in parallel with Fic1. (A) Diagram of Pxl1 to scale. LIM domains are shown as gray ovals, and PXXP motifs are depicted as asterisks. The site of the truncation used in the in vitro binding assay is indicated with an arrow. (B) Coimmunoprecipitation of Cdc15 with HA-Pxl1 was performed as in Fig. 4 D. The asterisk indicates a background band, and the arrow marks the HA-Pxl1 bands. IP, immunoprecipitation; WB, Western blot. (C) in vitro binding assay was performed as in Fig. 4 E. Binding is indicated with an asterisk. (D) GFP-pxl1 and cdc15_ΔSH3 GFP-pxl1 strains were grown at 25°C and imaged live. (E) Spores were germinated and imaged as in Fig. 8 (A–D). BF, bright field. Bars, 5 μm.