SH3 domains of Cdc15 and Imp2 function cooperatively in cytokinesis. (A) cdc15ΔSH3-FLAG–KanR and imp2ΔSH3-FLAG–HygR cells were crossed, and tetrads were pulled. Circles indicate missing double mutants, and the recovery rates are listed. (B) Images were taken for several resulting tetrads, and single colonies of each genotype are shown. wt, wild type. (C) Spores of the indicated genotypes were germinated overnight and imaged in the first cell division. Bright field images are overlaid onto GFP images. Below, bright field (BF) and GFP images of two germinating spores before (left) and after (right) initiation of septation are shown separately. (D) Spores were germinated as in C and imaged for both Imp2ΔSH3-GFP and Cdc15ΔSH3-mCherry localization. Bars, 2 μm.
