Figure 3. Dynamics of rings and ring... | Rockefeller University Press

Figure 3.

$Figure 3. Dynamics of rings and ring components in cdc15ΔSH3. (A) Kymographs were created from lines drawn across the division site in five videos for each rlc1-GFP and cdc15ΔSH3-FLAG3 rlc1-GFP. Time is depicted on the vertical scale bar. (B) Contractile ring event timing for each cell in A was determined and averaged (n = 5 each; Fig. S1 H, available). Timing of cytokinesis in wild-type (wt) cells was plotted on the y axis, and the time taken for corresponding events in wild-type and cdc15ΔSH3-FLAG3 cells was plotted on the x axis. (C) The time taken for wild-type and cdc15ΔSH3-FLAG3 cells to complete stages of cytokinesis was determined for each genotype, and differences were determined by Student's t tests. P-values and SEM (error bars) are included. (D) FRAP measurements of Cdc15-GFP or Cdc15ΔSH3-GFP signals are shown (n = 24 each). t1/2 and mobile fraction values were calculated from best-fit curves and are shown below. The difference in t1/2 is significant (P = 0.046), as is the difference in Fm (P = 0.0009). R2 values for wild-type and cdc15ΔSH3 curves are 0.9753 and 0.9752, respectively. (E) FRAP measurements of Rlc1-GFP in cdc15+ or cdc15ΔSH3-FLAG3 backgrounds (n = 28 each). t1/2 and mobile fraction values were calculated from best-fit curves. The differences in t1/2 and Fm are not significant (P = 0.651 and P = 0.589, respectively). R2 values for wild-type and cdc15ΔSH3 curves are 0.9365 and 0.9518, respectively.$

Dynamics of rings and ring components in cdc15_ΔSH3. (A) Kymographs were created from lines drawn across the division site in five videos for each rlc1-GFP and cdc15_ΔSH3-FLAG₃rlc1-GFP. Time is depicted on the vertical scale bar. (B) Contractile ring event timing for each cell in A was determined and averaged (n = 5 each; Fig. S1 H, available). Timing of cytokinesis in wild-type (wt) cells was plotted on the y axis, and the time taken for corresponding events in wild-type and cdc15_ΔSH3-FLAG₃ cells was plotted on the x axis. (C) The time taken for wild-type and cdc15_ΔSH3-FLAG₃ cells to complete stages of cytokinesis was determined for each genotype, and differences were determined by Student's t tests. P-values and SEM (error bars) are included. (D) FRAP measurements of Cdc15-GFP or Cdc15_ΔSH3-GFP signals are shown (n = 24 each). t_1/2 and mobile fraction values were calculated from best-fit curves and are shown below. The difference in t_1/2 is significant (P = 0.046), as is the difference in F_m (P = 0.0009). R² values for wild-type and cdc15_ΔSH3 curves are 0.9753 and 0.9752, respectively. (E) FRAP measurements of Rlc1-GFP in cdc15⁺ or cdc15_ΔSH3-FLAG₃ backgrounds (n = 28 each). t_1/2 and mobile fraction values were calculated from best-fit curves. The differences in t_1/2 and F_m are not significant (P = 0.651 and P = 0.589, respectively). R² values for wild-type and cdc15_ΔSH3 curves are 0.9365 and 0.9518, respectively.