Figure 4.
Figure 4. Localization of wild-type and bimC-box mutant KLP61F. Distribution of KLP61FT933A-GFP and KLP61FT933D-GFP point mutants compared with wild-type KLP61F-GFP during metaphase (A) and anaphase B (B) in embryos injected with rhodamine-tubulin to mark MTs. The mutants barely associate with spindle MTs. (C) Localization of wild-type KLP61F-GFP to spindles is MT dependent. (top) Prometaphase KLP61F-GFP embryos containing rhodamine tubulin were imaged, transferred to a microinjection microscope, and injected with 25 mg/ml colchicine. (bottom) 3D projections 3 min later, after transfer back to the spinning disc microscope, show that spindle MTs were depolymerized, which results in a complete loss of KLP61F-GFP. Bars, 5 μm.

Localization of wild-type and bimC-box mutant KLP61F. Distribution of KLP61FT933A-GFP and KLP61FT933D-GFP point mutants compared with wild-type KLP61F-GFP during metaphase (A) and anaphase B (B) in embryos injected with rhodamine-tubulin to mark MTs. The mutants barely associate with spindle MTs. (C) Localization of wild-type KLP61F-GFP to spindles is MT dependent. (top) Prometaphase KLP61F-GFP embryos containing rhodamine tubulin were imaged, transferred to a microinjection microscope, and injected with 25 mg/ml colchicine. (bottom) 3D projections 3 min later, after transfer back to the spinning disc microscope, show that spindle MTs were depolymerized, which results in a complete loss of KLP61F-GFP. Bars, 5 μm.

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