N-cadherin depletion mimics OL-pc expression. (A) Control U251 cells and OL-pc-HA transfectants were treated with control or N-cadherin siRNA, and their migration profiles during would healing were analyzed as described in Fig. 5. Time-lapse photographs and videos are available in Fig. S4 and Videos 8 and 9 . (B) Quantification of the instantaneous velocities of cells treated with control or N-cadherin siRNA. See Fig. 5 for the method of statistical analysis. n = 22 and 20 for control and OL-pc transfectants, respectively, treated with control siRNA; n = 19 and 20 for control and OL-pc transfectants, respectively, treated with N-cadherin siRNA. ***, P < 0.0005. n.s., P = 0.68. (C) The farthest and nearest indices obtained as in Fig. 5 D. n = 38 and 39 for control and OL-pc transfectants treated with control siRNA, respectively; n = 40 and 41 for control and OL-pc transfectants treated with N-cadherin siRNA, respectively. *, P < 0.05; **, P < 0.005. n.s., P = 0.56 and 0.13 for the farthest and nearest indices, respectively. Bar, 50 μm.