Nap1 and WAVE1 are required for OL-pc function. (A) Control U251 cells and OL-pc-HA transfectants were treated with control, Nap1, or WAVE1 siRNA, and their migration pattern during wound healing was analyzed as described in Fig. 5. See Fig. S4 for the original photographs of these cells. Also see Videos 6 and 7 for the videos of Nap1-depleted cells without and with OL-pc, respectively. The arrow indicates the direction of wound healing. (B) Quantification of the instantaneous velocities of those cells. n = 31 and 29 for control and OL-pc transfectants with control siRNA, respectively; n = 40 for both control and OL-pc transfectants with Nap1 siRNA; n = 29 and 34 for control and OL-pc transfectants, respectively, treated with WAVE1 siRNA. **, P < 0.005; ***, P < 0.0005. (C) The farthest and nearest indices obtained as in Fig. 5 D. n = 23 and 22 for control and OL-pc transfectants with control siRNA, respectively; n = 30 for both control and OL-pc transfectants with Nap1 RNAi; n = 23 and 24 for control and OL-pc transfectants with WAVE1 siRNA, respectively. *, P < 0.05; **, P < 0.005. n.s., P = 0.35 and 0.44 for Nap1 siRNA–treated cells and P = 0.83 and 0.06 for WAVE1 siRNA–treated cells for the farthest and nearest indices, respectively. Bar, 50 μm.