Figure 4. Bursting disassembly generates... | Rockefeller University Press

Figure 4.

$Figure 4. Bursting disassembly generates barbed ends not recognized by CapZ. Fluorescence images show Alexa 647 actin (left) and Alexa 488 CapZ (right) in filament bundles. Barbed ends are oriented toward to the right. The bar graph shows the CapZ/actin filament ratio, a measure of the fraction of capped barbed ends in the bundle. Data represent mean and standard deviation of eight filament bundles. (A) Filament bundles polymerized from L. polyphemus acrosomal fragments were incubated directly with Alexa 488 CapZ. (B) Filament bundles were partially disassembled with 8 μM cofilin for 90 s then incubated with CapZ. (C) Filament bundles were partially disassembled with 2.5 μM cofilin, 1.5 μM coronin, and 50 nM Aip1 for 25 s, then incubated with CapZ. (D) Filament bundles were partially disassembled with cofilin, coronin, and Aip1 as in C, incubated in buffer for 10 s, then incubated with CapZ. Bar, 1 μm.$

Bursting disassembly generates barbed ends not recognized by CapZ. Fluorescence images show Alexa 647 actin (left) and Alexa 488 CapZ (right) in filament bundles. Barbed ends are oriented toward to the right. The bar graph shows the CapZ/actin filament ratio, a measure of the fraction of capped barbed ends in the bundle. Data represent mean and standard deviation of eight filament bundles. (A) Filament bundles polymerized from L. polyphemus acrosomal fragments were incubated directly with Alexa 488 CapZ. (B) Filament bundles were partially disassembled with 8 μM cofilin for 90 s then incubated with CapZ. (C) Filament bundles were partially disassembled with 2.5 μM cofilin, 1.5 μM coronin, and 50 nM Aip1 for 25 s, then incubated with CapZ. (D) Filament bundles were partially disassembled with cofilin, coronin, and Aip1 as in C, incubated in buffer for 10 s, then incubated with CapZ. Bar, 1 μm.