The cytokinesis midbody is asymmetrically inherited by one daughter cell. (A and B) Hela or BSC1 cells were fixed and immunostained with α-MKLP1. (A and B) During cytokinesis (A), MKLP1 localizes to the midbody structure (periphery of the dividing cell outlined in white), and after completion of cytokinesis, MKLP1 is found in ring-like structures in interphase cells (B), which is shown in the high magnification inset (box width, 2 μm). (C) Quantification of percentage of interphase Hela and BSC1 cells with a midbody remnant by MKLP1 immunostaining (right y-axis, red; n = 355 Hela cells and n = 375 BSC1 cells). Error bars show SD. (D) Time-course gallery of midbody inheritance in BSC1 cells at completion of cytokinesis (Video 8). The midbody is indicated by arrows (time in minutes). (E) Time-course of midbody inheritance in Hela cells at completion of cytokinesis (Video 9). The midbody is indicated by arrows (time in minutes). Bars, 5 μm.