SDCM and FRAP analysis reveals that post-Golgi vesicles are directed to the cleavage furrow from both daughter cells and form accumulations of individual vesicles. (A) BSC1 cells expressing VSVG-YFP were imaged live by SDCM, with one confocal stack acquired every 5 s (Video 1). (B) Paths of 20 post-Golgi vesicles from each daughter cell were manually tracked (Video 2). (C) Individual paths of two vesicles (one from each daughter cell) are indicated with blue and red arrowheads and shown in RGB merge. Yellow arrowheads show highlighted vesicles in adjacent frames. (D) BSC1 cells expressing VSVG-YFP (green) were imaged by SDCM (one confocal stack every 10 s). Dashed rectangles indicate enlarged region shown in E. (E) Time-lapse fluorescence imaging reveals fluorescence recovery in photobleached ROI (dashed rectangles). Individual vesicles trafficking into bleached area from both daughter cells are denoted by blue and red arrowheads (Video 4). (F) Quantification of FRAP kinetics (asterisk demarks bleaching period of ∼3 s).