Figure 2.
Figure 2. Accumulation of VSVG-YFP–labeled post-Golgi exocytic membrane is distinct from other membrane compartments. (A and B) Hela cells expressing VSVG-YFP (green) were fixed 60 min after transfer to 32°C and immunostained with α–Lamp-1 for lysosomes (A, red) or α-EEA1 for early endosomes (B, red). (C) BSC1 cells expressing both transferrin receptor and VSVG-YFP (green) were pulse labeled with 5 mg/ml Texas red–conjugated transferrin (red) after transfer to 32°C and chased for 15 min with transferrin-free media before imaging by SDCM. Dashed rectangles indicate enlarged regions shown in adjacent panels.

Accumulation of VSVG-YFP–labeled post-Golgi exocytic membrane is distinct from other membrane compartments. (A and B) Hela cells expressing VSVG-YFP (green) were fixed 60 min after transfer to 32°C and immunostained with α–Lamp-1 for lysosomes (A, red) or α-EEA1 for early endosomes (B, red). (C) BSC1 cells expressing both transferrin receptor and VSVG-YFP (green) were pulse labeled with 5 mg/ml Texas red–conjugated transferrin (red) after transfer to 32°C and chased for 15 min with transferrin-free media before imaging by SDCM. Dashed rectangles indicate enlarged regions shown in adjacent panels.

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