Post-Golgi membrane accumulates in the cleavage furrow of cells undergoing cytokinesis. (A) BSC1 cells expressing VSVG-YFP were fixed 60 min after transfer to permissive temperature (32°C) and immunostained with α-tubulin (red). The number of cells with accumulations of VSVG-YFP (green)–labeled post-Golgi membrane in the furrow was evaluated. Boxed regions are enlarged in adjacent panels and show VSVG alone and VSVG/tubulin merge. G, Golgi; asterisks, VSVG EMMAs in the furrow. (B) Manual scoring of symmetric, asymmetrical, and no accumulation of VSVG-labeled membrane in the furrow was quantified in 100 cells. Error bars show SD.