Figure 2.
Figure 2. Antibody-mediated cross-linking results in down-modulation of surface AChR. CHO-K1/A5 (top) or C2C12 (bottom) cells were labeled with mAb 35 on ice, washed, and incubated with secondary AlexaFluor568 antibody (0 h) or were chased at 37°C for 6 h and incubated with secondary antibody on ice. The histograms represent the surface fluorescence values obtained by quantifying secondary AlexaFluor568 antibody fluorescence per cell normalized to 0 h. Bars represent the weighted mean of average from four experiments with at least 65 cells each (CHO-K1/A5) or from two experiments with at least 50 cells each (C2C12) ± SEM (error bars). *, P < 0.0001. Bars, 10 μm.

Antibody-mediated cross-linking results in down-modulation of surface AChR. CHO-K1/A5 (top) or C2C12 (bottom) cells were labeled with mAb 35 on ice, washed, and incubated with secondary AlexaFluor568 antibody (0 h) or were chased at 37°C for 6 h and incubated with secondary antibody on ice. The histograms represent the surface fluorescence values obtained by quantifying secondary AlexaFluor568 antibody fluorescence per cell normalized to 0 h. Bars represent the weighted mean of average from four experiments with at least 65 cells each (CHO-K1/A5) or from two experiments with at least 50 cells each (C2C12) ± SEM (error bars). *, P < 0.0001. Bars, 10 μm.

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