Figure 3.
Figure 3. PS1 and PS2 accelerate the clearance of cytosolic Ca2+ after entry via muscle nAChR channels. (A) Ca2+-dependent fluorescence changes (ΔF/Fo) after 300-ms hyperpolarizing pulses to induce Ca2+ entry through nAChR expressed in the plasma membrane. Traces show mean data from >35 recordings in multiple oocytes injected 3 d earlier with αβδγ nAChR subunits alone (control) or together with PS1, PS2, or SERCA2b cRNA. The inset shows the same data on a semilogarithmic scale. (B–D) Data from, respectively, control, PS2, and SERCA2b oocytes, fitted by biexponential decays (superimposed red traces). (E) Table listing fast (T1) and slow (T2) time constants of double-exponential fits to the Ca2+ decay data.

PS1 and PS2 accelerate the clearance of cytosolic Ca2+ after entry via muscle nAChR channels. (A) Ca2+-dependent fluorescence changes (ΔF/Fo) after 300-ms hyperpolarizing pulses to induce Ca2+ entry through nAChR expressed in the plasma membrane. Traces show mean data from >35 recordings in multiple oocytes injected 3 d earlier with αβδγ nAChR subunits alone (control) or together with PS1, PS2, or SERCA2b cRNA. The inset shows the same data on a semilogarithmic scale. (B–D) Data from, respectively, control, PS2, and SERCA2b oocytes, fitted by biexponential decays (superimposed red traces). (E) Table listing fast (T1) and slow (T2) time constants of double-exponential fits to the Ca2+ decay data.

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