Shootin1 RNAi does not affect the movement of polylysine-coated beads on axonal growth cones. (A and B) DIC micrographs showing retrograde movement of polylysine-coated beads on axonal growth cones (two days in vitro) expressing a control shRNA (A, left) or a shRNA against shootin1 (B, left), and a time series of the indicated areas at 30-s intervals (A and B, right). (C) The percentage of polylysine-coated beads that showed retrograde flow on axonal growth cones expressing the control (n = 46) or shootin1 shRNA (n = 49), mean velocity of moving beads as means ± SEM, and the percentage of moving beads with indicated velocities. Bar, 5 μm.