Shootin1 associates with L1. (A) Deconvolved images of an axonal growth cone stained by anti-shootin1 and anti-L1 antibodies. (inset) An enlarged view of the rectangle. Arrowheads indicate shootin1 accumulation in a filopodium. Bar, 5 μm. (B) Coimmunoprecipitation of shootin1 and L1 from P5 rat brain lysates. Brain lysates were incubated with anti-shootin1 antibody, anti-L1 antibody, or control IgG. The immunoprecipitates were analyzed by immunoblotting with anti-shootin1 and anti-L1 antibodies.