RasGEF Q regulation of myosin II assembly. (A) Aggregation-competent AX2 and gefQ⁻ cells were fixed and stained for myosin II. Images were taken with a confocal microscope. Every confocal section is accompanied by a pseudo-3D projection, in which the z axis represents the intensity of myosin II staining over the scanned area. Bar, 5 μm. (B) Myosin II levels in cytoskeletal ghosts from cells in vegetative and aggregation-competent stages. The graph (below) represents a mean of four independent experiments. The error bars indicate SD. (C) Lysates from vegetative wild-type AX2 and gefQ⁻ cells were subjected to 2D SDS-PAGE analysis for determining the phosphorylation status of myosin II.