Figure 5.
Figure 5. FH1FH2mDia2 stabilizes MTs against cold-induced depolymerization. (A) SDS gel analysis of the resistance of self-assembled MTs to cold-induced depolymerization after incubating with His- (His-mDia2) or GST- (GST-mDia2) tagged FH1FH2mDia2. After centrifugation, equivalent amounts of the pellets and supernatants were resolved by SDS-PAGE and visualized by Coomassie. (B) Immunostaining of MTs that survive cold-induced depolymerization in the presence of His-FH1FH2mDia2 (His-mDia2) or buffer alone. (C) SDS gel analysis of the resistance of self-assembled MTs to cold-induced depolymerization after incubating with wild-type and mutant His-FH1FH2mDia2. Pellets at 4°C and input material are shown. Panels shown are from a single gel. Bar, 10 μm.

FH1FH2mDia2 stabilizes MTs against cold-induced depolymerization. (A) SDS gel analysis of the resistance of self-assembled MTs to cold-induced depolymerization after incubating with His- (His-mDia2) or GST- (GST-mDia2) tagged FH1FH2mDia2. After centrifugation, equivalent amounts of the pellets and supernatants were resolved by SDS-PAGE and visualized by Coomassie. (B) Immunostaining of MTs that survive cold-induced depolymerization in the presence of His-FH1FH2mDia2 (His-mDia2) or buffer alone. (C) SDS gel analysis of the resistance of self-assembled MTs to cold-induced depolymerization after incubating with wild-type and mutant His-FH1FH2mDia2. Pellets at 4°C and input material are shown. Panels shown are from a single gel. Bar, 10 μm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal