Figure 1.
Figure 1. Spinophilin is expressed in the immune cells and its localization in DCs is dynamic. (a) Detection of spinophilin by Western blot in serial dilutions of cell lysates from brain, spleen, or DCs. Numbers below indicate the ratio of adjusted mean density of spinophilin to actin on the Western blot. (b) Localization of spinophilin was detected by immunofluorescence in immature (top) and mature BMDCs (bottom) as indicated. DCs were stained with anti-spinophilin (RU466; Allen et al., 1997) and anti-MHCII (14.4.4 anti–IE-k FITC) antibodies and, to detect lysosomes, anti-Lamp (Lamp2) antibodies. (c) Localization of spinophilin, actin, and MHCII was detected by immunofluorescence in immature (top) and mature BMDCs (bottom) as indicated. DCs were stained with anti-spinophilin (RU466; Allen et al., 1997), TRITC-phalloidin, and anti-MHCII (14.4.4 anti–IE-k FITC) antibodies. Images in section c were pseudo-colored for illustrative purposes. Bars, 5 μM.

Spinophilin is expressed in the immune cells and its localization in DCs is dynamic. (a) Detection of spinophilin by Western blot in serial dilutions of cell lysates from brain, spleen, or DCs. Numbers below indicate the ratio of adjusted mean density of spinophilin to actin on the Western blot. (b) Localization of spinophilin was detected by immunofluorescence in immature (top) and mature BMDCs (bottom) as indicated. DCs were stained with anti-spinophilin (RU466; Allen et al., 1997) and anti-MHCII (14.4.4 anti–IE-k FITC) antibodies and, to detect lysosomes, anti-Lamp (Lamp2) antibodies. (c) Localization of spinophilin, actin, and MHCII was detected by immunofluorescence in immature (top) and mature BMDCs (bottom) as indicated. DCs were stained with anti-spinophilin (RU466; Allen et al., 1997), TRITC-phalloidin, and anti-MHCII (14.4.4 anti–IE-k FITC) antibodies. Images in section c were pseudo-colored for illustrative purposes. Bars, 5 μM.

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